• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文OA文献 >First Report of Soybean Vein Necrosis Disease Caused by Soybean vein necrosis-associated virus in Wisconsin and Iowa
【2h】

First Report of Soybean Vein Necrosis Disease Caused by Soybean vein necrosis-associated virus in Wisconsin and Iowa

机译：威斯康星州和爱荷华州大豆静脉坏死相关病毒引起的大豆静脉坏死病的首次报道

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Several viral diseases of soybean (Glycine max) have been identified in the north-central U.S. soybean production area, which includes Wisconsin and Iowa (2). Previously, Soybean vein necrosis disease (SVND) caused by Soybean vein necrosis-associated virus was reported in Arkansas, Tennessee, and other southern states (4). In September 2012, soybean plants with symptoms similar to those reported for SVND (4) were observed in fields across Wisconsin and Iowa. Symptoms included leaf-vein and leaf chlorosis, followed by necrosis of the leaf veins and eventually necrosis of the entire leaf. Six samples with symptoms indicative of SVNaV were collected from research plots located at the West Madison Agricultural Research Station located in Madison, WI. An additional three samples were collected from three locations in central Iowa. Total RNA extracted from each sample using the Trizol Plus RNA purification kit (Invitrogen, Carlsbad, CA) was used to generate complementary DNA (cDNA) using the iScript cDNA synthesis kit (Bio-Rad Laboratories, Hercules, CA) following the manufacturers\u27 suggested protocols. The resulting cDNA was used as template in a PCR with SVNaV-specific primers, SVNaV-f1 and SVNaV-r1 (3). PCRs of two of the six Wisconsin samples and two Iowa samples were positive. Amplification products were not detected in the other five samples. The amplification products from the four strongly positive samples were purified using the Wizard SV Gel and PCR Purification Kit (Promega, Madison, WI) following the manufacturer\u27s suggested protocol and were subjected to automated sequencing (University of Wisconsin Biotechnology Center or Iowa State University, DNA Sequencing Facilities). BLASTn (1) alignments of the 915-bp consensus sequence revealed 98% and \u3e99% identity of the Wisconsin and Iowa samples, respectively, with the ‘S’ segment of the SVNaV ‘TN’ isolate (GenBank Accession No. GU722319.1). Samples from the same leaf tissue used above, were subjected to serological tests for SVNaV using antigen coated-indirect ELISA (3). Asymptomatic soybeans grown in the greenhouse were used as a source of leaves for negative controls. These tests confirmed the presence of SVNaV in eight symptomatic soybean leaflets collected in Wisconsin and Iowa. The asymptomatic control and one Iowa sample, which was also PCR-negative, were also negative by serological testing. Six additional samples from soybean fields in as many Wisconsin counties (Fond Du Lac, Grant, Green, Juneau, Richland, Rock) tested positive for SVNaV using specific primers that amplify the ‘L’ segment (4). The sequenced amplification products (297-bp) showed 99 to 100% homology to the L segment of the TN isolate (GU722317.1). To our knowledge, this is the first report of SVNaV associated with soybean and the first report of SVND in Wisconsin and Iowa. Considering that little is known about SVNaV, it is assumed that it is like other Tospoviruses and can cause significant yield loss (4). Soybean is a major cash crop for Wisconsin and Iowa, and infection by SVNaV could result in potential yield loss in years where epidemics begin early and at a high initial inoculum level.
机译：在美国中北部的大豆产区，发现了几种大豆病毒病（最大大豆），其中包括威斯康星州和爱荷华州（2）。以前，在阿肯色州，田纳西州和其他南部州报道了由大豆静脉坏死相关病毒引起的大豆静脉坏死病（SVND）（4）。 2012年9月，在威斯康星州和爱荷华州的田地中观察到症状与SVND（4）相似的大豆植物。症状包括叶脉和叶绿化，然后是叶脉坏死，最后是整个叶坏死。从位于威斯康星州麦迪逊市的西麦迪逊农业研究站的研究区收集了六种具有指示SVNaV症状的样品。从爱荷华州中部的三个地点收集了另外三个样本。使用Trizol Plus RNA纯化试剂盒（Invitrogen，Carlsbad，CA）从每个样品中提取的总RNA用于i厂家生产的iScript cDNA合成试剂盒（Bio-Rad Laboratories，Hercules，CA）\ u27建议的协议。所得的cDNA在SVNaV特异性引物SVNaV-f1和SVNaV-r1的PCR中用作模板（3）。六个威斯康星州样品和两个爱荷华州样品中的两个PCR呈阳性。在其他五个样品中未检测到扩增产物。按照制造商建议的方案，使用Wizard SV凝胶和PCR纯化试剂盒（Promega，Madison，WI）纯化来自四个强阳性样品的扩增产物，并进行自动测序（威斯康星大学生物技术中心或爱荷华州立大学） ，DNA测序设备）。 BLASTn（1）对915-bp共有序列的比对揭示了威斯康星州和爱荷华州样品分别与SVNaV'TN'分离物的'S'区段具有98％和\ u3e99％的同一性（GenBank登录号GU722319.1 ）。使用抗原包被的间接ELISA（3）对来自上述相同叶组织的样品进行SVNaV血清学测试。温室中生长的无症状大豆用作阴性对照的叶片来源。这些测试证实了在威斯康星州和爱荷华州收集的八种有症状大豆小叶中存在SVNaV。通过血清学检测，无症状对照和一个爱荷华州样本也呈PCR阴性，均为阴性。威斯康星州许多县（Fond Du Lac，Grant，Green，朱诺，Richland，Rock）大豆田的另外六个样品使用扩增“ L”部分的特异性引物测试为SVNaV阳性（4）。测序的扩增产物（297 bp）显示与TN分离物（GU722317.1）的L段有99％到100％的同源性。据我们所知，这是与大豆相关的SVNaV的首次报道，也是威斯康星州和爱荷华州的SVND的首次报道。考虑到人们对SVNaV知之甚少，因此认为它与其他Tospovirus病毒一样，可能会导致大量产量损失（4）。大豆是威斯康星州和爱荷华州的主要经济作物，在流行病开始较早且初始接种量很高的年份，SVNaV感染可能导致潜在的产量损失。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号